
Dr. Moïse Bendayan
Professor of Pathology & Cell Biology
Departement de Pathologie et Biologie Cellulaire Universite de Montréal
In cell biology, the high contrast imaging provided by a LVEM is a major advantage. Electron microscopy in general allows for the study of the activities, functions, properties and organization of cells. This provides a deep understanding of organization and function within cells.
Electron microscopy approaches complement light microscopy by providing greater detail. Electron microscopy is the only technique with sufficient resolution to localize proteins to small membrane subdomains within the cell. Researchers can better understand the location and organization of organelles, actin filaments in the cytoskeleton, and molecular complexes such as nuclear pores.
Although there are instances where staining is desirable for diagnostic purposes, the necessity to stain samples in order to generate adequate detailed contrast cannot be viewed as advantageous. The LVEM microscopes still allows for staining as an option, yet high contrast result are acquired from samples in their inherent, natural state. The LVEM25 has the added benefit of being able to work with conventionally prepared thin sectioned materials. This provides images without the side effects often encountered such as staining artifacts or the sample crashing out by chemical reaction with heavy metals.
Electron microscopy traditionally is one of the most demanding techniques to learn. However, the simplicity of the LVEM5 and LVEM25 combined with the elimination of the staining step makes it accessible to many more researchers.
The versatile LVEM5 electron microscope operates with four distinct imaging modes; TEM, SEM, STEM and ED. This provides for comprehensive imaging and finer study. The more powerful LVEM25 is built upon the same platform as the LVEM5 and offers the same benefits, together with higher beam energy.
Whether it's in the fields of pathology or other life sciences, researchers and medical professionals are assured of top notch imaging with LVEM microscopes
The LVEM5 in cell biology can be particularly useful both in routine microscopy and in research.
In cell biology and active morphological research of cellular and subcellular structures, this instrument opens an entirely new field. The low voltage and the high contrast that it generates allow for simplified fixation protocols. Fixation with osmium tetroxide is not required anymore to generate nice contrasted images of cell components. Furthermore, due to the fact that the electrons are not highly accelerated, penetration, scattering and transmission of electrons through the section are much more efficient and generate differences in contrasts within cell structures. Hence, substructures and heterogeneities unknown up to now can be revealed within cellular compartment that were considered homogeneous.
The instrument generates images that are identical to those generated by a conventional 80KV transmission electron microscope. The LVEM5 has the advantage of avoiding additional tissue treatments such as post-sectioning staining with uranyl acetate and lead citrate. Even without counterstaining, the tissue reveals the usual cellular structures as a standard 80KV transmission microscope due to the high contrast generated by the low voltage. Compared to standard electron microscopes, the LVEM5 has the advantage of reducing the time for tissue preparation together with avoiding several treatments with heavy metals
The LVEM5 carries a great potential for advancing our comprehension of cellular structures, composition and organization.
Professor of Pathology & Cell Biology
Departement de Pathologie et Biologie Cellulaire Universite de Montréal
LVEM5 Benchtop Electron Microscope
LVEM25 Electron Microscope
LVEM 25E Electron Microscope
LVEM 5
TEM
Bacteriophage
Negative stained section
LVEM 5
TEM
Bacteriophage
Negative stained section
LVEM 5
TEM
Tobacco Mosaic Virus
Stained particles on carbon film
Negative stained
LVEM 5
TEM
Tobacco Mosaic Virus
Stained particles on carbon film
Negative stained
LVEM 5
TEM
Tobacco Mosaic Virus
Stained particles on carbon film
Negative stained
LVEM 5
TEM
Tobacco Mosaic Virus
Stained particles on carbon film
Negative stained
LVEM 5
TEM
Tobacco Mosaic Virus
Stained particles on carbon film
Negative stained
LVEM 5
TEM
Tobacco Mosaic Virus
Stained particles on carbon film
Negative stained
LVEM 5
TEM
Tobacco Mosaic Virus
Stained particles on carbon film
Negative stained
LVEM 5
TEM
Tobacco Mosaic Virus
Stained particles on carbon film
Negative stained
LVEM 5
TEM
Kidney
Stained section
Point of interest: nucleus
LVEM 5
TEM
Kidney
Stained section
Point of interest: nucleus
LVEM 5
TEM
Kidney
Stained section
LVEM 5
TEM
Kidney
Stained section
LVEM 5
TEM
Kidney
Stained section
Point of interest: leukocyte in capilary
LVEM 5
TEM
Kidney
Stained section
Point of interest: leukocyte in capilary
LVEM 5
TEM
Kidney
Stained section
Point of interest: leukocyte in capilary
LVEM 5
TEM
Kidney
Stained section
Point of interest: leukocyte in capilary
LVEM 5
TEM
Kidney
Stained section
Fixed by OsO4, point of interest: section microtubules (citas) in various orientation
LVEM 5
TEM
Kidney
Stained section
Fixed by OsO4, point of interest: section microtubules (citas) in various orientation
LVEM 5
TEM
Kidney
Stained section
Fixed by OsO4, point of interest: section microtubules (citas) in various orientation
LVEM 5
TEM
Kidney
Stained section
Fixed by OsO4, point of interest: section microtubules (citas) in various orientation
LVEM 5
TEM
Kidney
Stained section
Fixed by OsO4, point of interest: section microtubules (citas) in various orientation
LVEM 5
TEM
Kidney
Stained section
Fixed by OsO4, point of interest: section microtubules (citas) in various orientation
LVEM 5
TEM
Murine Heart Muscle
Ultrathin section
Fixed by OsO4, point of interest: mitochondrias and muscle fibres
LVEM 5
TEM
Murine Heart Muscle
Ultrathin section
Fixed by OsO4, point of interest: mitochondrias and muscle fibres
LVEM 5
TEM
Murine Heart Muscle
Ultrathin section
Fixed by OsO4, point of interest: mitochondrias and muscle fibres
LVEM 5
TEM
Murine Heart Muscle
Ultrathin section
Fixed by OsO4, point of interest: mitochondrias and muscle fibres
LVEM 5
SEM
Pollen Grains (Forsythia)
Particles on carbon film
Gold coated
LVEM 5
SEM
Pollen Grains (Forsythia)
Particles on carbon film
Gold coated
LVEM 5
SEM
Pollen Grains (Forsythia)
Particles on carbon film
Gold coated
LVEM 5
SEM
Pollen Grains (Forsythia)
Particles on carbon film
Gold coated
LVEM 5
SEM
Pollen Grains (Forsythia)
Particles on carbon film
Gold coated
LVEM 5
SEM
Pollen Grains (Forsythia)
Particles on carbon film
Gold coated
LVEM 5
SEM
Pollen Grains (Narcissus)
Particles on carbon film
Gold coated
LVEM 5
SEM
Pollen Grains (Narcissus)
Particles on carbon film
Gold coated
LVEM 5
SEM
Pollen Grains (Narcissus)
Particles on carbon film
Gold coated
LVEM 5
SEM
Pollen Grains (Narcissus)
Particles on carbon film
Gold coated
LVEM 5
SEM
Butterfly
Particles on carbon film
Gold coated
LVEM 5
SEM
Butterfly
Particles on carbon film
Gold coated
LVEM 5
SEM
Butterfly
Particles on carbon film
Gold coated
LVEM 5
SEM
Butterfly
Particles on carbon film
Gold coated
LVEM 5
SEM
Butterfly
Particles on carbon film
Gold coated
LVEM 5
SEM
Butterfly
Particles on carbon film
Gold coated
LVEM 5
SEM
Butterfly
Particles on carbon film
Gold coated
LVEM 5
SEM
Butterfly
Particles on carbon film
Gold coated
LVEM 5
SEM
Horse Tail
Particles on carbon film
Gold coated
LVEM 5
SEM
Horse Tail
Particles on carbon film
Gold coated
LVEM 5
SEM
Horse Tail
Particles on carbon film
Gold coated
LVEM 5
SEM
Horse Tail
Particles on carbon film
Gold coated
LVEM 5
SEM
Horse Tail
Particles on carbon film
Gold coated
LVEM 5
SEM
Horse Tail
Particles on carbon film
Gold coated
LVEM 25
TEM
Retina Section
Stained section
LVEM 25
TEM
Retina Section
Stained section
LVEM 25
TEM
Retina Section
Stained section
Point of interest: photoreceptor cell with well-defined cell core
LVEM 25
TEM
Retina Section
Stained section
Point of interest: photoreceptor cell with well-defined cell core
LVEM 25
TEM
Retina Section
Stained section
Point of interest: photoreceptor cell with well-defined cell core
LVEM 25
TEM
Retina Section
Stained section
Point of interest: photoreceptor cell with well-defined cell core
LVEM 25
TEM
Retina Section
Stained section
Point of interest: section of a capillary containing erythrocytes in the upper part of the image
LVEM 25
TEM
Retina Section
Stained section
Point of interest: section of a capillary containing erythrocytes in the upper part of the image
LVEM 25
TEM
Nasal Epithelial Cells
Stained section
LVEM 25
TEM
Nasal Epithelial Cells
Stained section
LVEM 25
TEM
Cilia Cross-Sectioned Stained section
Nasal brushing chemically fixed by 2.5% glutaraldehyde.Point of interest: ultra-structure of ciliated cells
LVEM 25
TEM
Cilia Cross-Sectioned Stained section
Nasal brushing chemically fixed by 2.5% glutaraldehyde.Point of interest: ultra-structure of ciliated cells
LVEM 25
TEM
Cilia Cross-Sectioned Stained section
Point of interest: transversal crossection of cilia with well visible structure: 9 peripheral triplets and 1 cetral dublet of microtubuli
LVEM 25
TEM
Cilia Cross-Sectioned Stained section
Point of interest: transversal crossection of cilia with well visible structure: 9 peripheral triplets and 1 cetral dublet of microtubuli
LVEM 25
TEM
Cilia Cross-Sectioned Stained section
Point of interest: visible ultrastructure with apparent dynein arms
LVEM 25
TEM
Cilia Cross-Sectioned Stained section
Point of interest: visible ultrastructure with apparent dynein arms
LVEM 25
TEM
Mouse brain
Stained section Neural tissue
LVEM 25
TEM
Mouse brain
Stained section Neural tissue
LVEM 25
TEM
Mouse brain
Stained section Neural tissue including axons with and without myelin sheath
LVEM 25
TEM
Mouse brain
Stained section Neural tissue including axons with and without myelin sheath
LVEM 25
TEM
Mouse brain
Stained section Neural tissue
LVEM 25
TEM
Mouse brain
Stained section Neural tissue
LVEM 25
TEM
Mouse brain
Stained section
Stained section of a neural tissue surrounded with mitochondria-containing myelinated axon
LVEM 25
TEM
Mouse brain
Stained section
Stained section of a neural tissue surrounded with mitochondria-containing myelinated axon
LVEM 25
TEM
Myelinated Axons
Stained section
Nerve tissue contrasted by 1% LC, fixed by 1.5% potassium ferrocyanide
LVEM 25
TEM
Myelinated Axons
Stained section
Nerve tissue contrasted by 1% LC, fixed by 1.5% potassium ferrocyanide
LVEM 25
TEM
Mouse brain
Stained section
Neural tissue
LVEM 25
TEM
Mouse brain
Stained section
Neural tissue
LVEM 25
TEM
Kidney
Stained section
90nm section, block stained by UAc, post-fixed by OsO4
LVEM 25
TEM
Kidney
Stained section
90nm section, block stained by UAc, post-fixed by OsO4
LVEM 25
TEM
Kidney
Stained section
Epithel and endothel cells with apparent cell cores
LVEM 25
TEM
Kidney
Stained section
Epithel and endothel cells with apparent cell cores
LVEM 25
TEM
Kidney
Stained section
LVEM 25
TEM
Kidney
Stained section
LVEM 25
TEM
Kidney
Stained section
Epithel and endothel cells with apparent cell cores
LVEM 25
TEM
Kidney
Stained section
Epithel and endothel cells with apparent cell cores
LVEM 25
TEM
Kidney
Stained section
Point of interest: podocytes
LVEM 25
TEM
Kidney
Stained section
Point of interest: podocytes
LVEM 25
TEM
Kidney
Stained section
Point of interest: podocytes with details of pedicles
LVEM 25
TEM
Kidney
Stained section
Point of interest: podocytes with details of pedicles
LVEM 25
TEM
Kidney
Stained section
Point of interest: podocytes with details of pedicles
LVEM 25
TEM
Kidney
Stained section
Point of interest: podocytes with details of pedicles
LVEM 25
TEM
Kidney
Stained section
Point of interest: podocytes with details of pedicles
LVEM 25
TEM
Kidney
Stained section
Point of interest: podocytes with details of pedicles
LVEM 25
TEM
Kidney
Stained section
Point of interest: podocytes transsection showing cell nucleus in the proximity of erythrocytes in capillary
LVEM 25
TEM
Kidney
Stained section
Point of interest: podocytes transsection showing cell nucleus in the proximity of erythrocytes in capillary
LVEM 25
TEM
Kidney
Stained section
Point of interest: podocytes
LVEM 25
TEM
Kidney
Stained section
Point of interest: podocytes
LVEM 25
TEM
Kidney
Stained section
Point of interest: granular endoplasmatic reticulum location in the proximity of epithelial cell nucleus
LVEM 25
TEM
Kidney
Stained section
Point of interest: granular endoplasmatic reticulum location in the proximity of epithelial cell nucleus
LVEM 25
TEM
Kidney
Stained section
Point of interest: detail of granular endoplasmatic reticulum
LVEM 25
TEM
Kidney
Stained section
Point of interest: detail of granular endoplasmatic reticulum
LVEM 25
TEM
Kidney
Stained section
Point of interest: mitochondria
LVEM 25
TEM
Kidney
Stained section
Point of interest: mitochondria
LVEM 25
TEM
Kidney
Stained section
LVEM 25
TEM
Kidney
Stained section
LVEM 25
TEM
Kidney
Stained section
Point of interest: basal membrane of glomerulus
LVEM 25
TEM
Kidney
Stained section
Point of interest: basal membrane of glomerulus
LVEM 25
TEM
Kidney
Stained section
Point of interest: fingerprint structure
LVEM 25
TEM
Kidney
Stained section
Point of interest: fingerprint structure
LVEM 25
TEM
Kidney
Stained section
Point of interest: fingerprint structure
LVEM 25
TEM
Kidney
Stained section
Point of interest: fingerprint structure
LVEM 25
TEM
Kidney
Stained section
Point of interest: fingerprint structure
LVEM 25
TEM
Kidney
Stained section
Point of interest: fingerprint structure
LVEM 25
TEM
Kidney
Stained section
Point of interest: tubulus pathology
LVEM 25
TEM
Kidney
Stained section
Point of interest: tubulus pathology
LVEM 25
TEM
Kidney
Stained section
Point of interest: section of renal corpuscle with capillary and podocyte
LVEM 25
TEM
Kidney
Stained section
Point of interest: section of renal corpuscle with capillary and podocyte
LVEM 25
TEM
Kidney
Stained section
Point of interest: podocyte with details of pedicles
LVEM 25
TEM
Kidney
Stained section
Point of interest: podocyte with details of pedicles
LVEM 25
TEM
Kidney
Stained section
Point of interest: amyloid nephropathy
LVEM 25
TEM
Kidney
Stained section
Point of interest: amyloid nephropathy
LVEM 25
TEM
Kidney
Stained section
Point of interest: amyloid nephropathy
LVEM 25
TEM
Kidney
Stained section
Point of interest: amyloid nephropathy
LVEM 25
TEM
Kidney
Stained section
Point of interest: amyloid nephropathy
LVEM 25
TEM
Kidney
Stained section
Point of interest: amyloid nephropathy
LVEM 25
TEM
Kidney
Stained section
Point of interest: amyloid nephropathy
LVEM 25
TEM
Kidney
Stained section
Point of interest: amyloid nephropathy
LVEM 25
STEM 10kV
Mouse Liver
Stained section
1% SM/En Acetates. Point of interest: hepatocyte nucleus surrounded by droplets of fat
LVEM 25
STEM 10kV
Mouse Liver
Stained section
1% SM/En Acetates. Point of interest: hepatocyte nucleus surrounded by droplets of fat
LVEM 25
TEM
Mouse Liver
Stained section
1% SM/En Acetates
LVEM 25
TEM
Mouse Liver
Stained section
1% SM/En Acetates
LVEM 25
STEM 10kV
Mouse Liver
Stained section
1% SM/En Acetates Point of interest: mitochondria of hepatocyte surrounded by droplets of fat
LVEM 25
STEM 10kV
Mouse Liver
Stained section
1% SM/En Acetates Point of interest: mitochondria of hepatocyte surrounded by droplets of fat
LVEM 25
TEM
Mouse Liver
Stained section
Point of interest: mitochondria of hepatocyte surrounded by droplets of fat
LVEM 25
TEM
Mouse Liver
Stained section
Point of interest: mitochondria of hepatocyte surrounded by droplets of fat
LVEM 25
TEM
Mouse Liver
Stained section
1% SM/En Acetates
LVEM 25
TEM
Mouse Liver
Stained section
1% SM/En Acetates
LVEM 25
TEM
Lungs
Stained section
Epon embedded, 85 nm section, chemically fixed, with Uranyless/Pb staining and coated with 2nm carbon. Point of interest: cilia of epithel cells
LVEM 25
TEM
Lungs
Stained section
Epon embedded, 85 nm section, chemically fixed, with Uranyless/Pb staining and coated with 2nm carbon. Point of interest: cilia of epithel cells
LVEM 25
TEM
Lungs
Stained section
Epon embedded, 85 nm section, chemically fixed, with Uranyless/Pb staining and coated with 2nm carbon. Point of interest: cross-section of epithel cells with apparent multiple nuclei
LVEM 25
TEM
Lungs
Stained section
Epon embedded, 85 nm section, chemically fixed, with Uranyless/Pb staining and coated with 2nm carbon. Point of interest: cross-section of epithel cells with apparent multiple nuclei
LVEM 25
TEM
Lungs
Stained section
Epon embedded, 85 nm section, chemically fixed, with Uranyless/Pb staining and coated with 2nm carbon. Point of interest: cross-section of bronchioles with cilia
LVEM 25
TEM
Lungs
Stained section
Epon embedded, 85 nm section, chemically fixed, with Uranyless/Pb staining and coated with 2nm carbon. Point of interest: cross-section of bronchioles with cilia
LVEM 25
TEM
Lungs
Stained section
Epon embedded, 85 nm section, chemically fixed, with Uranyless/Pb staining and coated with 2nm carbon. Point of interest: cross-section of lungs tissue with visible basal membrane of epithel cell.
LVEM 25
TEM
Lungs
Stained section
Epon embedded, 85 nm section, chemically fixed, with Uranyless/Pb staining and coated with 2nm carbon. Point of interest: cross-section of lungs tissue with visible basal membrane of epithel cell.
LVEM 25
TEM
Lungs
Stained section
Epon embedded, 85 nm section, chemically fixed, with Uranyless/Pb staining and coated with 2nm carbon.Point of interest: cilia brush border
LVEM 25
TEM
Lungs
Stained section
Epon embedded, 85 nm section, chemically fixed, with Uranyless/Pb staining and coated with 2nm carbon.Point of interest: cilia brush border
LVEM 25
TEM
Lungs
Stained section
Epon embedded, 85 nm section, chemically fixed, with Uranyless/Pb staining and coated with 2nm carbon.Point of interest: granular endoplasmatic reticulum
LVEM 25
TEM
Lungs
Stained section
Epon embedded, 85 nm section, chemically fixed, with Uranyless/Pb staining and coated with 2nm carbon.Point of interest: granular endoplasmatic reticulum
LVEM 25
TEM
Lungs
Stained section
Epon embedded, 85 nm section, chemically fixed, with Uranyless/Pb staining and coated with 2nm carbon.Point of interest: endoplasmatic reticulum cisternae surrounding the cell nucleus
LVEM 25
TEM
Lungs
Stained section
Epon embedded, 85 nm section, chemically fixed, with Uranyless/Pb staining and coated with 2nm carbon.Point of interest: endoplasmatic reticulum cisternae surrounding the cell nucleus
LVEM 25
TEM
Lungs
Stained section
Epon embedded, 85 nm section, chemically fixed, with Uranyless/Pb staining and coated with 2nm carbon. Point of interest: granular endoplasmatic reticulum cisternae
LVEM 25
TEM
Lungs
Stained section
Epon embedded, 85 nm section, chemically fixed, with Uranyless/Pb staining and coated with 2nm carbon. Point of interest: granular endoplasmatic reticulum cisternae
LVEM 25
TEM
Lungs
Stained section
Epon embedded, 85 nm section, chemically fixed, with Uranyless/Pb staining and coated with 2nm carbon.Point of interest: mitochondria with visible cristae
LVEM 25
TEM
Lungs
Stained section
Epon embedded, 85 nm section, chemically fixed, with Uranyless/Pb staining and coated with 2nm carbon.Point of interest: mitochondria with visible cristae
LVEM 25
TEM
Lungs
Stained section
Epon embedded, 85 nm section, chemically fixed, with Uranyless/Pb staining and coated with 2nm carbon.Detail of GER: cisterns and fat droplets
LVEM 25
TEM
Lungs
Stained section
Epon embedded, 85 nm section, chemically fixed, with Uranyless/Pb staining and coated with 2nm carbon.Detail of GER: cisterns and fat droplets
LVEM 25
TEM
Lungs
Stained section
Epon embedded, 85 nm section, chemically fixed, with Uranyless/Pb staining and coated with 2nm carbon.Cross-section of lung cell: apparent granular endoplasmatic reticulum, cell nuclei and fat droplets
LVEM 25
TEM
Lungs
Stained section
Epon embedded, 85 nm section, chemically fixed, with Uranyless/Pb staining and coated with 2nm carbon.Cross-section of lung cell: apparent granular endoplasmatic reticulum, cell nuclei and fat droplets
LVEM 25
TEM
Lungs
Stained section
Epon embedded, 85 nm section, chemically fixed, with Uranyless/Pb staining and coated with 2nm carbon. Point of interest: cilia brush border
LVEM 25
TEM
Lungs
Stained section
Epon embedded, 85 nm section, chemically fixed, with Uranyless/Pb staining and coated with 2nm carbon. Point of interest: cilia brush border
LVEM 25
TEM
Lungs
Stained section
Epon embedded, 85 nm section, chemically fixed,with UAc/Pb staining and coated with 2nm carbon. Point of interest: mitochondria
LVEM 25
TEM
Lungs
Stained section
Epon embedded, 85 nm section, chemically fixed,with UAc/Pb staining and coated with 2nm carbon. Point of interest: mitochondria
LVEM 25
TEM
Lungs
Stained section
Epon embedded, 85 nm section, chemically fixed, with Uranyless/Pb staining and coated with 2nm carbon. Point of interest: cross-section of free cell in lung tissue
LVEM 25
TEM
Lungs
Stained section
Epon embedded, 85 nm section, chemically fixed, with Uranyless/Pb staining and coated with 2nm carbon. Point of interest: cross-section of free cell in lung tissue
LVEM 25
TEM
Muscle
Unstained section
Structure of muscle cell: visible Z-lines, actin and myosin filaments
LVEM 25
TEM
Muscle
Unstained section
Structure of muscle cell: visible Z-lines, actin and myosin filaments
LVEM 25E
EDS
Immunology
LVEM 25E
EDS
Immunology
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