Dr. Moïse Bendayan
Professor of Pathology & Cell Biology
Departement de Pathologie et Biologie Cellulaire Universite de Montréal
LVEM FOR
Cell Biology
In cell biology, high contrast imaging provided by a LVEM is a major advantage. Electron microscopy allows for the study of the functions and organization of cells.
Unlike light-based techniques, electron microscopy offers the resolution needed to localize proteins within small membrane subdomains. Researchers can clearly visualize organelles, cytoskeletal filaments, and molecular complexes like nuclear pores.
LVEM systems produce high-contrast images without staining, saving time and avoiding common artifacts. Staining is still supported when needed, but it’s no longer a requirement for detailed contrast when using LVEM. The LVEM 25E also accepts conventionally prepared, thin-sectioned samples, allowing researchers to skip harsh chemicals that can distort structures or trigger unwanted reactions.
Electron microscopy traditionally is one of the most demanding techniques to learn. However, the simplicity of the LVEM 5 and LVEM 25E combined with the elimination of the staining procedures makes it accessible to many more researchers.
The versatile LVEM 5 benchtop electron microscope operates with four distinct imaging modes; TEM, SEM, STEM and ED. This provides for comprehensive imaging and finer study. The more powerful LVEM 25E is built upon the same platform as the LVEM 5 and offers the same benefits, together with higher beam energy.
Whether in pathology, molecular biology, or broader life sciences, LVEM brings high-quality, lab-ready imaging to teams who need precision without complexity.
LVEM5 User Testimonial
The LVEM5 in cell biology can be particularly useful both in routine microscopy and in research.
In cell biology and active morphological research of cellular and subcellular structures, this instrument opens an entirely new field. The low voltage and the high contrast that it generates allow for simplified fixation protocols. Fixation with osmium tetroxide is not required anymore to generate nice contrasted images of cell components. Furthermore, due to the fact that the electrons are not highly accelerated, penetration, scattering and transmission of electrons through the section are much more efficient and generate differences in contrasts within cell structures. Hence, substructures and heterogeneities unknown up to now can be revealed within cellular compartment that were considered homogeneous.
The instrument generates images that are identical to those generated by a conventional 80KV transmission electron microscope. The LVEM5 has the advantage of avoiding additional tissue treatments such as post-sectioning staining with uranyl acetate and lead citrate. Even without counterstaining, the tissue reveals the usual cellular structures as a standard 80KV transmission microscope due to the high contrast generated by the low voltage. Compared to standard electron microscopes, the LVEM5 has the advantage of reducing the time for tissue preparation together with avoiding several treatments with heavy metals
The LVEM5 carries a great potential for advancing our comprehension of cellular structures, composition and organization.
Application Notes
NANOSCALE FROM YOUR BENCHTOP
LVEM5 Benchtop Electron Microscope
ALL IN ONE EM
LVEM 25E Electron Microscope
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