LVEM 25E Accessories

Image

Norcada UltiFlow

TEM grid pre-treatment device

Norcada’s UltiFlow is an efficient pre-treatment solution for TEM grids, preparing up to twelve grids at once by activating and hydrophilizing their surfaces. This improves sample adhesion, reducing the sample amount needed and minimizing the risk of sample separation or droplet formation.

Notable benefits of the UltiFlow include:

  • Simple 3-step operation: load your sample, input process time & power, and press start for results within minutes
  • Less expensive and easier to maintain than plasma or glow discharge systems
  • No plasma, chemicals, process gases, pump down, or vacuum required
  • Portable, small, and lightweight (4 lbs, 9" x 6" x 3")
Image
Image
Image

Pre-Treatment Comparison

TEM imaging showed that wide-band light-treated grids using the Norcada UltiFlow device resulted in well-distributed nanoparticles with reduced agglomeration compared to untreated grids. For Gold Nanoparticles (AuNPs), the treatment of illuminating the grids with this combination of wavelengths performs similarly to Glow Discharge, achieving good dispersion with minimal clumping. Polymer particles showed even better results on UltiFlow-treated grids, displaying a more homogeneous distribution than those on Glow Discharge-treated grids. The grids were also not damaged as opposed to samples prepared by Glow Discharge.

Scale bars measure 200 nm.

Tilt Holder

The LVEM 25E transmission electron microscope mode can be used in combination with the optional tilt holder to perform Electron Tomography. This is a technique for obtaining detailed 3D structures from 2D images. In the process, a beam of electrons is passed through the sample at incremental degrees of rotation around the center of the target sample. This information is collected and used to assemble a three dimensional image of the target.

Additionally, the LVEM 25E scanning electron microscope mode can be used in combination with the optional tilt holder to perform photogrammetry. This technique involves extraction of 3D geometry information from 2D images taken from a sample held at different angles relative to the BSE detectors.

The Tilt Holder for the LVEM 25E allows analysis of various different sample types from different points of view, thus enabling reconstruction of a 3D image of the sample.

Key Specifications
  • ±20º of tilt

  • Compatible with TEM, SEM and STEM modes

ASBESTOS FIBER
Image
TEM GRID
Image
INSECT LEG
Image